Sentence examples for by assembling sequences from from inspiring English sources
By assembling sequences from large genomic clones we have determined that RRH localises within an interval of approximately 110 kb between EGF and IF on chromosome 4q25.
By assembling sequences from two plates, the coverage doubled to an average of 6.4×, the number of contigs (average 9.9) was reduced, the assembly included over 95 kbp in contigs, and the full length scaffold had over 98% coverage relative to the reference PGB04 assembly.
This step eliminated duplicated loci that have diverged in the flanking regions (except recent duplicates) and reduced null alleles by assembling sequence data from regions with coverage ≥1× for anchoring primers.
By assembling sequence information for a single gene region from all species, in contrast to the usual focus of large scale genomics projects which acquire sequence information for all genes in single taxa, DNA barcodes can provide a quick preview of recent evolutionary history [28].
This was performed by assembling transcript sequences from the GenBank, RefSeq and Ensembl databases from the transcribed regions of the genome.
The clusters obtained by assembling EST sequences from GenBank and our ESTs were mined for SNPs using a pipeline that integrates six freely available SNP discovery tools: SNPserver [ 48], PolyBayes [ 49], Quality SNP [ 50], PolyFreq [ 51], MiraEST [ 52], and ssahaSNP [ 53].
A total of 48,300 (66.3%) unique sequences with a significant match were annotated (Table 2).> -wrap-foot> The annotations were obtained by comparing the assembled sequences with sequences from KEGG, Nr, and UniProt of public databases.
The assembled sequences obtained from pooled reads were validated by sequence based alignments against ESTs of P. kurrooa submitted at NCBI dbEST by our group.
We acquired the complete plastid-genome sequence of C. reinhardtii strain CC-503 by assembling ptDNA sequences collected from the C. reinhardtii Whole Genome Shotgun Reads Trace Archive Database at GenBank.
A total of 90.8 Mb of assembled sequences was obtained from 18.22× coverage sequences generated by 454 GS FLX-Titanium (454 Life Science, Roche).
BmCR1B element sequence was reconstructed by assembling of partial sequences from different contigs.
