Therefore, the gene cg0156 and its encoded protein were analysed by means of growth assays of a defined deletion mutant strain, transcriptional studies, and electrophoretic mobility shift assays (EMSAs) to identify the regulon, binding sites, and effectors of this predicted transcriptional regulator.
A recovery assay was also performed by adding a defined amount of MDA before incubating with the oxidant mixture.
Tang, D. et al. Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay.
Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined "wound field".
Saturation curves were defined by assaying enzyme activity at different concentrations of the variable substrate or effector with saturating levels of the other components.
By assaying several β-carboline compounds, we define certain chemical groups that modulate the affinity of this class of compounds for inhibition of tau phosphorylation.
We expressed these constructs in the TVCs using a defined Foxf enhancer15, and assayed migration quantitatively.
In conclusion, anti-NF155 antibodies measured by high-specificity assays defined a distinct subset of CIDP patients presenting with younger onset age, tremor, extremely high CSF protein levels, symmetric spinal root and plexus hypertrophy, and marked prolongation of distal and F-wave latencies, and therefore might be a biomarker for a unique subset of CIDP.
CXCR2 neutralization did not influence JHMV viral loads within either the brain or spinal cord during chronic infection, as determined by measuring viral titers by plaque assay at defined times post-treatment (Figure 2B).
Spectroscopic methods in combination with labeling and competition assays now define a mechanism conserved from archaea to humans.
