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The proof-of-concept was validated by assay of polyamidoamine (PAMAM) dendrimers that were fortified in human urine samples.
Cells were characterised by assay of alkaline phosphatase, detection of type 1 collagen, and production of osteocalcin.
The results were normalized for transfection efficiencies by assay of β-galactosidase activity.
The supernatant was percleared with protein G sephearse beads (Sigma) followed by assay of total protein (BCA kit, Pierce).
The quantity of different states of Ure2 entering into cells was determined by assay of glutaredoxin enzymatic activity, as described previously [22].
The alloantigen specificity of the CTL lines was determined by assay of interferon-γ (IFN-γ) production in response to stimulation with KI-LCL cells, as described previously [24], [25].
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Expression and surface localization of laccases in target cells were confirmed by assays of Western blot, immunofluorescence microscopy and flow cytometry.
The cytotoxicity and transforming activity of nickel subsulfide, nickel oxide and nickel sulfate was studied by assays of colony-forming efficiency and of transformation of rat tracheal epithelial (RTE) cells to enhanced growth variants (EGVs) and immortal growth variants (IGVs).
The production of infectious virus in cells transfected with pLAI was confirmed by assays of the cell culture supernatant using the P4R5 indicator cell line (data not shown).
Lipase production was followed by assaying of the lipase activity in the supernatant as described in enzyme assays.
Second, to identify if adjunct aripiprazole will improve bone turnover as measured by assays of osteoblastic and osteoclastic activity.
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