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The relation between compounds and targets is defined by assay data from the Discovery Platform.
We have probed site-directed mutants by assay and by deuterium exchange.
Furthermore, it was shown that the inhibitory potencies are not affected significantly by assay ionic strength.
This table gives log10 mean and log10 variance by assay plate, bacterial origin, resistance status, treatment, and number of replicates.
The conflicting data could be explained by assay variation [82 84] and differences in timing of measurements.
The proof-of-concept was validated by assay of polyamidoamine (PAMAM) dendrimers that were fortified in human urine samples.
Cells were characterised by assay of alkaline phosphatase, detection of type 1 collagen, and production of osteocalcin.
The binding improvement over the amine-coupling system shown by assay was confirmed by a separate surface plasmon resonance experiment.
The similar behavior of candidate compounds in structure and activity space and their opposite behavior in functional efficacy space was assessed by assay related target similarity (ARTS) method.
Measurements from such studies can be influenced by assay design and ligand-specific characteristics, both of which may contribute to discrepancies in literature reports.
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Thresholds to annotate inhibitors and non-inhibitors were applied in an assay-by-assay fashion, trying to minimize incoherence between multiple measurements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com