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Relative expression was determined by applying the comparative Ct method, as described previously[ 23].
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Data quantification was performed by applying the modified comparative Ct method [62].
Then, these models with the crystallographic structures suggested by BLAST were used as template for applying the comparative modelling strategy in order to obtain the complete models for both regions.
When applying the comparative CT method, one histological normal thyroid tissue was used as calibrator sample.
By applying the approach for a comparative analysis between countries, it can also provide recommendations to enhance the effectiveness of RE-related policies for regional economic development.
The levels of IGFBP3 expression in cell lines were calculated as a fold change from a calibrator sample (normal prostate cell line PWR-1E) using the Comparative CT method by applying the formula 2−ΔΔ C T (Livak and Schmittgen, 2001).
This study uses a comparative research design by applying the same multidimensional model of urban park access to community-level survey data collected in the cities of Brisbane Australiaandand Zhongshan (China).
It presents a comparative study between results obtained by applying the proposed system and that obtained by applying the most recent existing system [22].
Thus, comparative EST-SSRs studies will be more reliable when the EST data sets are analysed by applying the same bioinformatics procedure.
For quantification of gene expression we applied the comparative Ct method, which was normalised by applying a geometric mean of 3 endogenous housekeeping genes (18 s rRNA, β actin and cyclophillin) [ 57].
Thus, further assessment work is needed to fully apply the comparative analysis of 'futures mapping processes' as suggested by Kuusi et al.[25, 26, 36].
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