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Discharge records were used to construct 86 Hospital service areas (HSA) by applying methods described in earlier publications[ 6, 7].
Because not all individuals were genotyped, both individual performance and progeny means were used for genomic evaluation by applying methods described by [ 12].
Sample preparation, analyses by the ethyl chloroformate NEFA method and GC, were performed by applying methods described in detail by Amer et al.. Quantification of ethyl chloroformate-NEFA esters was obtained using external calibration curves for each NEFA.
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Applying methods described by Lu and Ades 17 and Dias, 18 we estimated a treatment effect to be the median value from the posterior distribution of odds ratios generated from a random effects simulation model, assuming a level of between-study heterogeneity observable in the available data.
We produced receiver-operating characteristic (ROC) curve plots and calculated the area under the ROC curve (AUC) by applying the methods described by Chambless et al (2011).
The results in Table 3 are obtained by applying the methods described above.
Relative expression ratios of miRNAs were calculated by applying the method described by Pfaffl et al (24).
Relative expression ratios of SUMO1/sentrin-specific peptidase 5 (SENP5) (Mm00769150_m1 and Hs01090047_m1; Applied Biosystems) and protein kinase Cδ (PRKCD) (Mm00440891_m1 and Hs01090047_m1; Applied Biosystems) mRNAs were calculated by applying the method described by Pfaffl et al (24).
FWHM was determined by applying the method described in detail by Hofheinz et al. [17].
By applying the method described in the previous section, the data were converted into momentum transfer space, giving one plot which describes the diffraction recorded across all pixels (Figure 4).
A good result was then obtained by applying the method described above to this data set using a filtering width of 3 h and a weight distance of 2 km (Figure 5B).
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