Sentence examples for by applying increasing concentrations from inspiring English sources

To further characterize the nature of ocular fluid as an inhibitor of VEGF-heparin binding, a dose-binding curve was plotted by applying increasing concentrations of VEGF165 dissolved in PBS or porcine ocular fluid to heparin-coated wells (Figure 5D).

Following bicuculline/MK801 and a subsequent wash, we examined the changes of Ca2+i by applying increasing concentrations of NMDA.

Antibodies were isolated from egg yolk using a standard procedure of step precipitation of proteins by applying increasing concentrations of polyethyleneglycol (PEG precipitation) as described earlier (Grebenchtchikov et al, 2002).

The protein was eluted by applying increased concentrations of NaCl in a Tris buffer devoid of EDTA.

Half maximal effective concentrations (EC50 values) of dopamine for activity inhibition were calculated from individual cells by applying increasing dopamine concentrations (0.001 100 µM, Sigma), each for 3 min. Mean frequency at each concentration was normalized to the respective baseline frequency for each cell.

The effect of Zn2+ was assessed by co-applying increasing concentrations of Zn2+ together with ACh (at its EC20 concentration, 120 and 50 µM ACh for the 9∶1 and 1∶9 α∶β ratios, respectively).

After observation of the vasospasm, trapidil was locally applied in increasing concentrations (10−5 10−4 M).

The Fz8-CRD-Fc protein was applied at increasing concentrations to the Wnt3a-producing cells.

In contrast, UROtsa cell viability was not significantly affected by the increasing concentrations of frankincense oil until 1 600 dilution was applied to the cells.

This binding was inhibited by increasing concentrations of unlabeled hPL (half-maximal concentrations, 2.2 and 3.4 nmol/L, respectively).

Native oligosaccharides and oxidized sugars (4-ketoaldose) were separated by applying a gradient with increasing concentration of sodium acetate over time as described by Westereng et al. [ 34].