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Third, by applying deconvolution techniques the image quality additionally improves.
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In addition, Tsunakawa et al. (1999) reconstructed the geomagnetic field variability during the M B polarity transition by applying a deconvolution technique using a continuous paleomagnetic record from the Kokumoto Formation.
10) and the Aβ-positive staining was detected using an automated image analysis system by applying a color deconvolution method [ 45] within the Hue, Saturation, Intensity (HSI) model (Color Deconvolution algorithm, Aperio Technologies, Inc., settings: hue value and width = 0.1 and 0.3, respectively, and saturation threshold = 0.04).
The site effect was also demonstrated by applying a coda deconvolution method to small earthquake data recorded on the ground surface and at a borehole station (Sawazaki et al. 2009) and by investigating repeating earthquakes (Rubinstein and Beroza 2004).
Thus, it is possible, by applying a spectral deconvolution analysis, using spectral forms of Chl and carotenoids in protein environment [ 20] to deduce the protein environment in which a carotenoid is bound.
Because Hi-net sensors have a natural frequency of 1 Hz and a relatively smaller sensitivity to lower frequency components, the sensor sensitivity at the stations is corrected by applying a time-domain digital deconvolution filter (Maeda et al. 2011).
Delineation of the cell boundary on each plane was achieved by applying two sequential filtering steps, a two-dimensional, nearest-neighbor deconvolution step to increase the signal-to-noise ratio of the boundary while at the same time evening out the local fluctuations in fluorescence signal, followed by a smoothening Gaussian 2-dimensional radial transformation (radius = 1 pixel).
Mean residue ellipticity calculations and secondary structure deconvolution analysis were performed on the DICHROWEB platform [37] by applying the SELCON3 algorithm [38].
Highest resolution confocal images were restored by three-dimensional (3D) deconvolution using Huygens Professional software, (Scientific Volume Imaging, http://www.svi.nl/) by applying classic maximum-likelihood estimation algorithm and automatically generated point-spread function.
The deconvolution of the last equation is performed using singular value decomposition (SVD) [45], a model-independent nonparametric deconvolution analysis with the potentiality of reducing the noise contribution by applying a suitable threshold to a singular value matrix.
Highest-resolution confocal images were restored by three-dimensional (3D) deconvolution using Huygens Professional software (Scientific Volume Imaging, Hilversum, Netherlands, http://www.svi.nl/) by applying classic maximum-likelihood estimation algorithm and automatically generated point-spread function.
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