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As well, subcellular metabolite distributions can be calculated, usually by applying a two- or three-compartmental calculation strategy [10], [11].
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The samples were eluted by applying a three steps gradient at 5, 10.5 and 100% of buffer B (20 mM sodium acetate, 3 M sodium chloride, pH 7.4) in 3 column volumes, at a flow rate of 3 mL/min and collected in 2 mL fractions.
This was followed by a six-minute interval of LVt ventilation, at the end of which lung mechanics (total pulmonary input impedance Zp by forced oscillation technique) were measured by applying an eight second oscillatory flow waveform at the airway opening.
The free induction decays (FID) for solution-state 31P-NMR spectra were transformed by applying a fourfold zero filling and a line broadening of 6 Hz.
The assembly was tightly sealed with a nitrile NBR-70 O-ring (M Seals A/S) by applying a pressure between two plates using four screws and plugged into a multiplexing control unit.
Maintain lens by applying a synthetic wax three to four times a year.
In this work we have generated a dataset with different degrees of zipper artifacts by applying a combination of three different CI algorithms with two AA algorithms.
It has been reported recently that imperceptible and robust audio watermarking can be achieved by applying a cascade of two different transforms on the original audio signal.
This has been achieved by applying a mixture of two different surfactants as stabilizing agents, that is, 4%% w/w of each polyoxyethylene glycerol trioleate and Tween 20®.
We used a new approach by applying a combination of two high-resolution techniques, namely microtomographic (microCT) imaging and histological serial semithin sectioning, providing the basis for subsequent three-dimensional reconstructions.
The short-term survival cutoff (1.5 years post-relapse survival) was defined retrospectively by applying a model of two normal distributions, in order to capture the visually apparent survival distribution, using the mixtools package in R [ 11].
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