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Scrape loading was performed with a razor blade by applying a cut on the cell monolayer, and the LY was then added to the cells.
We illustrated the potential of monitoring using ADM by applying a cut off value of 70 pg/mL, which is close to the 99th percentile of the normal range (43 pg/mL): patients who were admitted with ADM levels above 70pg/mLL had a 28-day survival rate of 55%.
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After adjustment for re-entrainment effects, by applying a cut-off filter to the outlet droplet size spectra, the experimental and theoretical outlet Sauter mean diameters show very good agreement.
By applying a cut-off filter, we found that only 58 of the 107 array targets displayed hybridization intensities that were above background levels in one or more of the seven treatments.
By applying a cut-off criterion of Q-value <0.05, the enrichment analysis revealed a few important pathways that were significantly enriched in response to Cd stress (Table 3).
The ORFs were used to query non-redundant databases using BLAST similarity searches, by applying a cut-off of 70% sequence identity over at least 80% of the ORF length.
Statistically significant differentially expressed genes from both microarray and RNA-seq data were obtained by applying a cut-off threshold of FDR ≤ 0.05 (5%) and an absolute log2 fold-change ≥ 0.6.
For a null treatment effect, the treatment estimates obtained by applying a cut-off of 80% were less biased than those obtained by directly analysing patients on the observed degree of compliance.
The resulting data set was further reduced by applying a cut-off fold change of 2 or greater, which led to a final set of 3,352 significantly changed probe sets.
By applying a momentum cut of 1 2 GeV, the data rate can be reduced by an order of magnitude.
The Monitor deal would reduce hospitals' income by £1.2bn by applying a 1.9% cut to tariff payments, forcing them to save 3.8% of their entire budget by being more efficient – a target hospitals say is completely unrealistic.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com