Sentence examples for by application of buffer from inspiring English sources

Bound proteins were eluted from the column by application of buffer A plus 600 mM NaCl, fractions of 1.5 ml were collected, and the protein compositions of the fractions were analyzed by SDS-PAGE (Laemmli, 1970); gels were stained with Coomassie Brilliant Blue R-250.

Then, samples were applied to glass fiber filter columns and centrifuged for 30 s followed by application of washing buffers provided by the kit and according to the manufacturer's instructions.

Elution of the viral particles was achieved by application of high salt buffer.

Prior to TRIzol extraction, alginate beads were washed in 0.15 M NaCl and dissolved by application of alginate dissolving buffer (55 mM sodium citrate (Na3C6H5O7), 30 mM Na ethylenediamine tetraacetic acid and 0.15 M NaCl at pH 6.8) for 15 minutes at 37°C followed by digestion of extracellular matrix in 0.06% w/v collagenase type 1 (Sigma) for 30 minutes.

Elution was achieved by application of three 2 ml aliquots of buffer C (buffer B supplemented with 250 mM imidazole).

The PVA-coated capillary was trimmed to ∼40 cm, installed in the instrument, and initially aligned by flushing a solution of 5 × 10 9 M fluorescein and 5 × 10 8 M 5-TAMRA in the SH buffer by application of pressure to the inlet.

We used the pH-nose of the Ca2+-current in bipolar cells and cones to determine whether the inhibition of the endogenous buffer system by application of benzolamide was effective.

The spectra were corrected by subtracting the buffer baseline followed by application of a smoothing function in Spectra Manager v2.0 (Jasco).

Rmin, Rmax and β were calculated by application of 10 μM ionomycin in either Ca2+-free buffer (1 mM EGTA) or in HBSS (1.3 mM Ca2+).

Pure monomeric MAβ is subsequently obtained by application of the denatured peptide to size exclusion chromatography (SEC) using native running buffer, e.g., 20 mM sodium phosphate, 50 mM sodium chloride, pH 7.2.

Total RNA was isolated from Bt CDC272 by incubation of cells in 100 μl TE buffer containing 1000 U lysozyme for 15 min at room temperature, followed by application of the miRNeasy kit (QIAGEN).