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For this reason, and also because commercially available kits for Q-PCR-based DNA quantification still need standardization [49] while increasing cost, it seems important to determine the degree of DNA fragmentation and to identify the unfavorable samples that need special attention in the interpretation of genotyping results by any molecular method.
Wild-type expression levels of genes at the HML and HMR loci are 1000-fold lower than the expression levels of the same genes when at the MAT locus, and efforts to detect expression of HML and HMR by any molecular method have shown the expression signal is indistinguishable from background noise.
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A total of 51 isolates were successfully identified by the molecular method.
Meanwhile, original plants of non- camellia tea were identified by using molecular method.
Similarly, routine abattoir inspection failed to detect 44.8% (47/105) of infected carcasses (as confirmed by culture and microscopy) and 37.5% (9/24) (confirmed by molecular method) (Table 3).
RPWM 2/2 earlier identified as Penicillium janthinellum but by molecular method was identified as Penicillium pinophilum.
Respiratory samples at the time of intubation were assessed by culture, by indirect immunofluorescence assay or by molecular methods in systematic tracheobronchial aspirates.
The species found by cultivation were confirmed by molecular methods.
Identification of Burkholderia spp. by the Phoenix EpiCenter should be confirmed by molecular methods and by the API 20NE system in suspected cases of B. pseudomallei infection.
Bhd. and authenticated by morphology and molecular methods by experts in the Mushroom Research Centre, University of Malaya, Malaysia.
These organisms can be monitored on surfaces by culture, molecular methods, or metabolic assays.
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