Sentence examples for by another three washes from inspiring English sources

The strips were then washed three times in TTBS, followed by another three washes in Tris buffer without Tween-20.

After three washes in PBS, quenching was performed in 50 mM ammonium chloride in PBS for 10 min, followed by another three washes in PBS.

Followed by another three washes, blots were visualized by Millipore Luminata Crescendo Western HRP substrate and developed on Amersham Hyperfilm ECL.

After three washes with PBS for 2 min each, horseradish peroxidase-streptavidin (Santa Cruz Biotechnology) was added to the section for 30 min, followed by another three washes, 2 min each with PBS.

After three washes, 2 min each with PBS, horseradish peroxidase-streptavidin (DAKO) was added to the section for 30 min, followed by another three washes, 2 min each with PBS.

After three washes, 2 min each with PBS, horseradish peroxidase-streptavidin (Santa Cruz Biotechnology) was added to the section for 30 min, followed by another three washes, 2 min each with PBS.

Plates were washed four times in PBS-T and incubated with 100 μl ExtrAvidin alkaline phosphatase conjugate (1 20000; Sigma E2636) for 2 hours, followed by another four washes.

The beads were then washed twice with buffer containing 25 mM Tris buffer (pH 8.0), 10% glycerol, 1 mM EDTA, 500 mM NaCl, 1 mM PMSF, 1 mM DTT, 1% Triton X-100, followed by another six washes with buffer containing 25 mM Tris buffer (pH 8.0), 10% glycerol, 1 mM EDTA, 150 mM NaCl, 1 mM PMSF, 1 mM DTT, 0.1% Triton X-100.

Next, 100 μl EDC and 100 μl NHS were added and the beads were rotated for 10 min at room temperature followed by another two washing steps with 0.5 ml NaAc buffer (pH 4.5) [ 45] and 5 min of rotation each.

Background reduction was accomplished with a 10-minute wash in acetylation buffer (0.1 M triethanolamine, 0.25% acetic anhydride), followed by another three DEPC-treated PBS washes of 5 minutes each.

Following incubation, cells were removed by two distilled H20 washes followed by three washes with 0.05 % Tween-PBS.