We first categorized the genes expressed in differentiating cerebellar neurons by analyzing the expression array data on the basis of combination of two factors: induction rate during differentiation and susceptibility to ICRF-193.
We tested this possibility by analyzing the expression level of RAD51 in PTEN-depleted DU145 or PTEN-knockout PC3 cells.
To verify this result, we used an alternative marker of autophagy by analyzing the expression of Atg5-containing protein complexes during development.
By analyzing the expression of a lacZ/Madr2 fusion protein, we find Madr2 confined to the nucleus in the deep, anterior cells of the second axis, whereas in epidermal and more posterior cells the protein is cytoplasmically localized.
Here, we sought to validate an experimental design to accurately predict the time of death by analyzing the expression of hundreds of upregulated genes in two model organisms, the zebrafish and mouse.
The myogenic differentiation potential of dt versus wild-type cells was monitored by examining the expression of myosin heavy chain by immunofluorescence, and by analyzing the expression profiles of myogenic regulatory factors and myogenic differentiation markers by RT-PCR.
The onset of osteoblast formation was evaluated by analyzing the expression of alkaline phosphatase (ALP).
We also evaluated the induction of senescence in these cells by analyzing the expression patterns of senescence and stemness marker genes.
Apoptosis test results were evaluated by analyzing the expression of typical apoptic markers such as cleaved caspase-3, full length caspase-3, and apaf-1 in western blot assay.
By analyzing the expression variations of the Eigen genes from different conditions, we had identified two, three, and five gene modules as cold-, heand, and salt-specific modules, respectively.
We characterized C5 and B10 cells by analyzing the expression of marker genes for embryonic stem cells and extraembryonic lineages.
