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These results should be confirmed in the future by analyzing levels of both proteins in hES cells after BMP treatment.

Potential effects of cannabinoid receptor antagonism on either necrosis or apoptosis were determined by analyzing levels of lactate dehydrogenase (LDH) in cell culture supernatants and by performing the Guava Nexin™ apoptosis test on AM251- and AM630-treated PSC.

Transfection efficacy was evaluated after 48 hours by analyzing levels of ZAP-70 mRNA and protein in total thymocytes by RT-PCR and western-blot, respectively (Figure 4A, upper panel).

By analyzing levels of PADI4 and cAT, as well as the levels of known tumor markers, we aimed to explore the pathogenic role of PADI4 during carcinogenesis.

Because we used a TLR7/8 ligand to stimulate the moDC, we analyzed levels of TLR7 by flow cytometry and real-time quantitative PCR.

Because Oligonol did not affect the mRNA levels of hormone sensitive lipase (HSL), we evaluated whether Oligonol regulated the activity of HSL by analyzing the levels of phosphorylated HSL in total cell lysates by performing western blotting.

Blockade of Wnt-1 signaling in both experiments was confirmed by analyzing intracellular levels of Dishevelled-3 and of cytosolic β-catenin.

We next assessed the general metabolic activity of mitochondria by analyzing the levels of ATP in Os-treated cells at different time points.

The extent of LPO was measured by analyzing the levels of TBARS in the gastric mucosa according to the previous method [ 25] but with minor modification.

Furthermore, we demonstrated the specificity of the amplified RNA pool by analyzing expression levels of different positive and negative gene markers using microarray hybridization and qRT-PCR.

As mTOR kinase represents a central player in autophagy regulation, we next tested the effect of ML-9 on autophagy by analyzing the levels of endogenous LC3-II protein, the most widely used marker for autophagosomes.