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Elemento's lab has already built a machine-learning model that predicts whether a patient has thyroid cancer by analyzing expression levels of specific genes.
Microarray data were confirmed by analyzing expression levels of 17 representative genes using reverse transcription quantitative polymerase chain reaction (RT-qPCR).
Furthermore, we demonstrated the specificity of the amplified RNA pool by analyzing expression levels of different positive and negative gene markers using microarray hybridization and qRT-PCR.
This was further examined by analyzing expression levels of sensitive virus responsive genes (VRGs) in head kidney and cardiac tissues.
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By analyzing expression level with microarray technique, 31 differentially expressed platelet-derived miRNAs have been confirmed and were regarded as promising biomarkers for noninvasive prediction of UC cases, ultimately revealing that miR-188-5p miR-188-5p miR-188-5piR-500, miR-422a5p, miR-378-5p, and miR-500 were dysregulated [ 59].
Therefore, we analyzed expression level changes by comparison to NIPs using a B. rapa microarray database.
We pursued this notion in a subset of patient samples (seven with low MYBL2 expression and two with normal MYBL2 expression, together with three controls) by analyzing the expression levels of mir-29a and mir-30e.
By analyzing gene expression levels and the composition of the carotenoids, it was found that the Psy and Lcyb genes had low expression levels and capsanthin synthesis decreased (the capsanthin content of the TRV/ Psy/ Lcyb group was far lower than that of the WT group) when the Psy and Lcyb genes were silenced simultaneously, which meant that the pepper fruit color was yellow.
By analyzing gene expression levels and the composition of the carotenoids, it was found that when the Ccs gene had a low expression level and the capsanthin synthesis decreased, the pepper fruit color was yellow when the Ccs gene was silenced.
The consistency was evaluated by analyzing the expression levels of: i) genes essential for growth, ii) non-essential genes with low expression that are involved in reactions with non-zero flux, and iii) genes with high expression values that are only involved in the catalysis of the reactions with predicted zero flux.
Further, by analyzing the expression levels of known estrogen-regulated genes (Naciff et al. 2003), we determined that there is not a significant difference in the relative expression level of any of those genes between animals exposed to Purina 5001 or casein-based diets.
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