Data from labeled samples were acquired using a BDTMLSRII cytometer (BD Biosciences) followed by analysis using the FlowJo software (Tree Star).
Monocyte-PEC cocultures with or without CD4+ cells were followed by analysis using fluorescence-activated cell scanning (FACS).
DNA extracted from each sample was analyzed followed by analysis using 95 primers of RAPD and 59 primers of ISSR to investigate genetic variation among samples.
The naupliar musculature of B. improvisus was stained with phalloidin to visualise F-actin, followed by analysis using confocal laser scanning microscopy (CLSM) with subsequent application of 3D imaging software.
Testicular levels of cytochrome P450 scc mRNA transcripts on 30, 60 and 90 days of treatment with the same dose were quantitated by biplex RT-PCR using β Actin as internal control followed by analysis using GelPro Analysis software.
The method of selection by analysis using precisely specified inputs and the well-established design methods of modern engineering, drawing on databases of materials and their attributes is elaborated.
Adsorption of As is normally evaluated by conducting batch adsorption experiments, followed by analysis using hydride-generation atomic absorption spectrophotometry (HGAA) or inductively coupled plasma-optical emission spectrometry (ICP-OES).
In this work, a method using a flux chamber coupled to solid phase micro-extraction (SPME) fibers followed by analysis using gas chromatography/mass spectrometry was developed to quantify emissions of six VFAs (acetic acid, propanoic acid, butanoic acid, pentanoic acid, hexanoic acid and 3-methyl butanoic acid) from non-enteric sources.
The major bioconversion products were confirmed by analysis using FT-IR, UV, GC, and GC-MS.
At the indicated time points, cells were immunostained with anti-TOM20 antibody and stained with DAPI followed by analysis using fluorescence confocal microscopy.
The calibration curve was obtained by preparing the standard solutions of a different concentration of a range of 1 50 ppm followed by analysis using a spectrophotometer.
