Sentence examples for by analysis on a from inspiring English sources
SYTO 59 was added to the erythrocyte suspension, followed by analysis on a cell microarray chip.
RNA concentrations were measured by spectrophotometry and integrity was ensured by analysis on a 1% agarose gel before proceeding with cDNA synthesis.
Quantitation was performed using Alexafluor 532 anti-mouse or Alexafluor 488 anti-rabbit secondary antibodies (Molecular Probes) followed by analysis on a Fujifilm Fluor Imager FLA-5100.
The size, quantity and quality of the resulting libraries were confirmed by analysis on a 2100 Bioanalyzer (Agilent) using a DNA 1000 chip before the library was diluted and used for emulsion PCR.
The frequency of parasite-specific CD8+ TCR-Tg T cells was determined by staining of single cell suspensions with anti-CD8-APC (clone 53 6.7) and anti-Thy-1.1-PE (clone His51, BD Biosciences), followed by analysis on a BD FACSCalibur BD Biosciencess).
After starvation 5×105 cells were fixed with BD™ Cytofix buffer (10 minutes on ice), permeabilized with BD™ Phosflow Perm Buffer III (30 minutes on ice), washed with BD Pharmingen™ Stain Buffer+FBS and stained with the Alexa Fluor® 647 mouse anti-Stat5 (pY694) antibody (30 minutes at room temperature, in the dark), followed by analysis on a BD FACSAria™.
The concentration of the pre-adenylated adaptor was estimated by analysis on an 15% denaturing polyacrylamide gel (made with American Bioanalytical Sequel NE, #AB13021, AB13022) and comparison of band intensity to known quantities of synthesized oligonucletides.
RNA quality was assessed by analysis on an Agilent 2100 bioanalyzer (Agilent, Palo Alto, CA).
DNA sequencing of both strands was performed by GENterprise Genomics (Mainz, Germany), followed by analysis on an ABI Prism 310 automated Genetic Analyzer (Applied Biosystems).
Band intensities were quantitated by analysis on an Apple computer using the public domain NIH Image program (developed at the U.S. National Institutes of Health and available on the Internet at http://rsb.info.nih.gov/nih-image/).
Purity of the VHH fusion protein was determined by analysis on an overloaded, Coomassie-stained, SDS-PAGE.
