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Until quite late in the 20th century, the mapping of human chromosomes presented a particularly difficult problem: experimental crosses could not be arranged in humans, and only a few linkages could be determined by analysis of unique family histories.
Quantification of K48-linked and K63-linked polyubiquitin was performed by analysis of unique diglycine signature peptides associated with each modification (41).
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By analysis of the unique gene reads and the unique exon reads, an RPKM value of 0.3 was set as the threshold for detectable gene expression above background.
By analysis of the unique variations of two samples, we found that the unique variations of SNPs and indels were located in 2847 and 2452 genes for the Wh3 and 2868 and 2613 genes for the Wh6 except for the intergenic annotation position.
By WGS analysis of unique paired samples, we here provide a comprehensive description of the genomic features of smoldering stages of MM, as well as models of their evolution to MM.
We thank David Hunter, Penn State University, for advice on the statistical analysis of unique substitutions.
The specific PCR primers for amplification of M. avidus peptidase genes were designed from the unique sequences obtained by analysis of ESTs using the OLIGO 5.0 software (National Bioscience) (Table 1) and the expected sizes of PCR products are listed in Table 2.
By BLASTN analysis of Nipponbare unique probes, 69,255 probes were confirmed to be SFPs against the 93-11 genome.
In this procedure, the cell wall carbohydrates are analyzed by gas chromatography tandem mass spectrometry (GC/MS/MS) of alditol acetates, the peptidoglycan by GC/MS (mass spectrometry) analysis of the unique amino acid diaminopimelic acid (after derivatization with isopropyl chloroformate), and the mycolic acids by liquid chromatography (LC)/MS (negative ion) without derivatization.
Yonezawa H, Kuramitsu HK: Genetic analysis of a unique bacteriocin, Smb, produced by Streptococcus mutans GS5.
The approach entailed the identification and analysis of 24 unique tetranucleotides that were defined by 8 consensus sequences.
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