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Cell surface antigen or ALDH expression was examined by counterstaining cells with 2 µg/ml PI followed by analysis of cells initially gated to eliminate cellular aggregates and necrotic material by forward and orthogonal light scatter and PI exclusion quantification.
There was no overlap between CD133+ and CD44+/CD24- populations, as determined by analysis of cells after triple staining (Additional file 3 [panels A and B]).
The low abundance of the INB during G1 was confirmed by analysis of cells after nocodazole release (data not shown) and is therefore unlikely to be an artefact of the CCE.
The present results clearly indicate that administration of BRB into cultures of A549 cells undergoing premature senescence reduced the development of senescent phenotype as revealed by analysis of cells morphometric features, activation of SA-β-gal and induction of CDK inhibitor p21WAF1.
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The mechanism of action of compound 9j was further investigated by analysis of cell apoptosis and cell cycle.
The importance of optimizing the ultrasonication is illustrated by analysis of cell disruption kinetics and DNA fragmentation.
In this experimental study BM-MSCs were cultured and the cells characterized by analysis of cell surface markers using flow cytometry.
Using an established transient miRNA screening protocol, the effects of miR-17, miR-92a and cluster miR17-92a on CHO growth and protein productivity were studied and followed by analysis of cell pools with stable overexpression of these miRNAs.
To investigate the oncolytic capacity and the induction of cell death of RV, PV and NDV the glioblastoma cell line U87 was simultaneously infected with two viruses followed by analysis of cell survival by MTT-assay and the type of cell death by FACS staining for Annexin V and Propidium iodide.
Toxicity on gram-negative bacteria (Escherichia coli), gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), and pathogenic yeast (Candida albicans) was evaluated by analysis of cell morphology, assessment of cell viability using the PrestoBlue assay, analysis of cell membrane integrity using the lactate dehydrogenase assay, and reactive oxygen species production.
The withdrawal of feeders/conditioned medium induced cell differentiation into trophectoderm derivatives, predominately trophoblast giant cells (Fig. 2E), which were detected by analysis of cell morphology and expression of genes specific for differentiated mouse TS cells (see below).
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