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By analysis of binding energy (BE) values, the feature of binding between carbon and oxygen is confirmed.
Fine epitope mapping by analysis of binding sequences identified in phage display experiments suggested that a peptide ligand containing the amino acid motif QPDRS might constitute part or all of the F1-40 epitope.
Katzen et al. [ 20] recently reported that insertion of EmrE into a discoidal membrane scaffold in correctly folded state during cell-free synthesis by analysis of binding activity of its substrate, tetraphenylphosphonium.
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By analysis of the binding energies in the spectra, it can be deduced that the samples consisted of Fe2O3, Ni2O3 and FeCl3.
This finding was supported by analysis of the binding pocket consensus of the promiscuous PDZ cluster, which revealed the preference of a histidine residue at position 14 (αB1, present in 80% of the binding pockets) and a valine at position 16 (αB5, present in 70% of the binding pockets; Fig. 4C panel b).
The conclusion is further supported by analysis of Cdc45 binding to the break site (Supplementary Fig S1C).
Forty-eight hours post-transfection, G418 (0.8 mg/ml) was applied and after approximately 21 days, individual G418-resistant colonies were selected and individual pure clonal stable cell lines/isolates were examined for TP expression by analysis of radioligand binding.
And the screening is conducted by the evaluation of molecular mechanics energy, and the potential protein 'hits' can be selected by further analysis of binding competitiveness against other ligands that bind to the same receptor site [ 9].
This is further supported by the analysis of binding at the Antennapedia (Antp) Hox gene where we find Ubx and Hth binding at multiple peaks across the gene in haltere disc chromatin and also a similar binding profile for Hth in the wing disc (Figure 7).
Usually, allosteric regulation of enzymes is studied by either analysis of binding or inhibition, and rarely is the pH dependence of either or both investigated.
The KD of the interaction was calculated to be 7.3 µM±1.5 µM by analysis of the equilibrium binding data from two separate experiments (shown in Fig. 4B) using the Langmuir binding model.
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