To assess the stem progenitor cell potential of the SP cells compared to the NSP cells, CDβ cells were stained using Hoechst dye 33342, followed by FACS analysis and sorting of each population.
The gating strategy for the analysis and sorting by FACS of different SC populations in this study is presented in Figure 1.
Conducting a Q study generally involves three consecutive steps: developing the statement set, identifying participants and conducting Q sort interviews, followed by analysis and interpretation of the Q sorts.
FACS analysis and sorting were performed by using the BD Aria or Fusion (BD Biosciences, San Jose, CA).
Analysis and sorting were performed by using a FACSVantage TurboSort SE (BD Biosciences).
In this work, we overcome all these issues with a novel method for generating isolated fungal microcolonies after the germination of microencapsulated spores, which makes possible their analysis by flow cytometry and sorting.
The open-ended questions in the exit interviews were typed into Excel, coded, and classified by themes and sorted for analysis.
Neurons from the mdDA system were isolated for expression analysis by FACS sorting (Fig. 11C) following previously described procedures (Jacobs et al., 2009b).
We have created a convenient, label-free, and function-based protease assay, suitable for flow cytometry analysis and cell sorting, by utilizing sensitive short-lived fluorescent substrates expressed in Escherichia coli (E. coli).
An inductive analysis was supported by the use of QSR NVivo 7 software for qualitative analysis, by sorting out specific themes that occurred in the interview transcripts.
