Expression levels of MAP4 isoforms in various tissues were compared by exporting and analysing published Affymetrix mouse exon array data sets (Pohl et al., 2009, see Supplementary file 2) from the Genome Browser.
The chemical structures were established using NMR spectroscopic, mass-spectral analyses, and published data.
Current global studies are meta-analyses of published data.
We therefore based our analyses by screening these published data for genes that might also be relevant for developmental processes of the forebrain.
Further analyses by integration of published data on carbohydrate content of plant tissues reveals that these changes in the plant ionome are likely due to dilution by the enhanced accumulation of carbohydrates observed when plants are grown in elevated CO2.
As well as validation above, RT-qPCR was used to further analyse genes/pathways identified by microarray and published data as potentially interesting, including lipid metabolism, xenobiotic and oxidative stress, and apoptosis.
For certain cancer sites, the secular analyses of mortality were extended back to 1911 by use of published data.
Results have been analysed and compared to published data.
Response: The "Results" do not just state the fact of the widely conserved promoter and its distance from the gene (which is indeed evident from Table 1) but also comparisons of the orthologous gene promoters supported by the alignment analyses and interpretations of published data.
Only public use files and published data were analysed.
