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Interestingly, by analysing its expression profile in breast cancer patients, DKK1 appears in tumours with a poor outcome, specifically hormone-independent cases.
In addition, clinical significance of SERPINB3 in tumour samples was assessed by analysing its expression in relation to time to recurrence, where early recurrence was defined as HCC reappearance within 24 months from radical resection.
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To exclude the possibility that impaired migration was due to deficiencies in CCR7 expression on Jak3−/− cells, we analysed its expression by FACS.
To evaluate the expression pattern of SYCP3 in human primary tumours, we analysed its expression by using an mRNA array that contained two duplicated spots of mRNA from 47 different tumours and 47 normal tissues from unmatched donors (supplementary Fig S2A and Table S1 online).
In an attempt to explore the expression and significance of miR-7 in NSCLC carcinogenesis, we first analysed its expression by real-time qRT PCR in human NSCLC cell lines including A549, H1299, H1355, and H460, in which it was greatly downregulated in each compared with the control normal HBE cells.
Therefore, by analysing protein expression from VEGF-treated and untreated renal cancer tissue, it may be possible to identify and validate protein biomarkers.
To evaluate the prognostic potential of serum CCL2 (sCCL2) and serum TNF-α (sTNF-α) in nasopharyngeal carcinoma (NPC) before treatment by analysing the expression of these two markers.
The osteoblastic phenotype of the cells was confirmed by analysing the expression of bone-specific genes (Runx2, osteocalcin, osteopontin, and osteonectin) by semiquantitative reverse transcriptase polymerase chain reaction (PCR).
We confirmed effective ablation of this subset in Nkx2.1 Cre Dbx1DTA embryos by analysing dbx1 expression at E9.5 (19ss; Fig. S3C, D).
We checked this point by analysing the expression of msgn1 (mesogenin-1, mespo) and dusp6 (mkp3).
CXCR3 internalisation was measured by analysing surface expression of CXCR3 by flow cytometry on the migrated NK cells.
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