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In Figure 1, the data are shown without normalization by an internal control RNA.
We normalized the values by an internal control, ribosomal protein large subunit 3 (RpL3 ).
The samples were quantified by comparison with a standard calibration curve created at the same time and the data was normalized by an internal control (GAPDH).
The ratio of renilla luciferase (RL, encoded by a reporter transcript bearing each 3'UTR of interest) to firefly luciferase (FL, encoded by an internal control reporter transcript) was set as 1 for the parent vector (psiCHECK2).
Recently, it has been suggested that the production of ROS and antioxidant scavengers may be tightly regulated by an internal control mechanism, involving uncoupling proteins and antioxidants (Brand et al. 2004).
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Conclusions: By developing the HSVgD-dPCR we have enhanced the diagnostic utility of real-time detection of HSV by incorporating an internal control reaction and by accurately typing a greater proportion of HSV positive specimens.
Reporter activity was corrected by cotransfecting an internal control plasmid RSVLuc (500 ng) or protein levels measured by Bradford Assay.
The method has been improved by using an internal control in parallel in a duplex reaction (27 ).
The method was modified by including an internal control, using primers targeting the 16S rDNA genes, in a multiplex PCR assay (Edwards et al. 1989).
Cutoff values of the composite scores for abnormal expression were determined by using an internal control consisting of 62 normal lung cores from 152 lung cancer blocks.
By importing an internal control redox probe into the sensing platform, we have overcome a disadvantage of electrochemical DNA sensors, namely, irreproducibility, and have done so without loss of sensitivity or selectivity.
More suggestions(15)
by an additional control
by an internal haemorrhage
by an internal gas
by an internal review
by an internal navigation
by an internal calibration
by an inefficient control
by an optimal control
by an internal power
by an internal chemistry
by an internal scroll
by an internal split
by an internal appointment
by an internal circadian
by an internal auditor
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