Sentence examples for by an integrating vector from inspiring English sources

This ex vivo approach typically relies on permanent modification of the cell genome by an integrating vector such as a retrovirus or plasmid DNA (that only rarely integrates but can be enriched by selecting cells with drugs).

Expression of ppk1 under a tetracycline-inducible promoter was achieved by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette.

Complementation of oppA was achieved by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette.

Complementation was therefore carried out with oppDA by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette.

The strains transformed with an integrating vector were checked by PCR with primers amplifying the corresponding promoter and reporter gene; whereas recombinant strains with replicating vectors were confirmed by recovering the plasmid after transformation into E. coli.

Moreover, we evidence that hENPs transduced with a non-integrating lentiviral vector to express Ngn2 induce similar recovery than those transduced with an integrating vector.

The documented cytotoxicity of the transposase, when constitutively expressed by an integrating retroviral vector (iRV), has been circumvented by the transient delivery of SB100X using retroviral mRNA transfer.

Here we present a new integrating vector derived from S. venezuelae phage SV1 [ 14, 15].

We also included 11 human ESC samples to establish a baseline and 20 iPSC lines derived from the above somatic cell types, including 15 lines we derived previously by using integrating vectors and 5 iPSC lines derived by using episomal vectors in this study.

While vaccinia virus and adenoviral vectors transduce hepatocytes effectively, the vectors are non-replicating and remain episomal, and thus gene expression is transient (Ilan et al. 1999; Li et al. 1993), by contrast, most integrating vectors into the host genome require a round of cell division for the integration event to occur (Ferry et al. 1991; Yamashita and Emerman 2006).

They appear distinct from other iPSCs derived by integrating vectors from either blood CD34+ cells or MSCs/fibroblasts.