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For immunohistochemistry, paraffin sections were stained after microwave treatment by an intact method.
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A MR spectroscopy method is described for the simultaneous discrimination and observation of sodium from the three compartments created by an intact cell monolayer.
All females were mated once by an intact adult male.
We believe medial suture fixation, enhanced by an intact lateral periosteal hinge, provides a mechanically robust fixation and thus a step cut is deemed unnecessary, eliminating the stress riser effect associated with this method.
To confirm whether Ser and Ser sites are phosphorylated in intact cells by an alternative method (i.e. immunoblot with phosphorylation site-specific antibodies) and if so, whether their levels are modulated by the presence of PCTAIRE-1, we transfected WT or mutant cyclin Y (S100A and S326A) with or without co-expression of WT PCTAIRE-1.
Hcy was determined by an immunoassay method.
The first paper dealing with analysis of an intact glycoprotein by a label-free EC method based on EIS was published in 2006.
Automated sorting of intact wheat kernels was achieved by an NIR spectroscopic method that could differentiate samples at a 60 mg/kg DON threshold, which was correct 96%% of the time [ 25].
We describe in this report an efficient method for generating human iPSCs with an intact genome by using a single episomal vector to transfect briefly-cultured adult PB or CB MNCs.
For example, the intact LTR retroelements (full-7) in cluster LTR_CE8 from C. elegans (see our companion website), which was also identified by an homology searching method in previous studies [ 14], was missed by step 1 in our method.
The aa sequences of protease domains containing an intact catalytic triad as identified by the sequence alignment were phylogenetically analyzed using the maximum likelihood (ML) method.
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