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In addition, two proteins transported by the T6SS are disrupted: a partial VgrG homolog is fused to a transposon subunit (ASA_2455), although a complete vgrG gene is encoded on pAsa4 (ASA_P4G080), and Hcp, which is encoded on pAsa4 (ASA_P4G082), is interrupted by an insertion sequence into which the Tn 21 element has inserted.
They i) were bordered at both sides by an insertion sequence (ISAba1); ii) were identical indicating that the amplification of the pld copies is rather recent; iii) presented a slippery sequence (GGGGG AAC CUU) resulting in a +1 frameshift analogous to the one found in the prfB gene of E. coli.
d csRNA gene is truncated by an insertion sequence.
In Pto DC3000, hopAG1 (PSPTO_0901) has been disrupted by an insertion sequence (IS) element.
The fadD10 coding region represents a pseudogene as it is disrupted by an insertion sequence.
Two copies of blaNDM-1 were detected and separated by an Insertion Sequence Common Region (IS CR) element.
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One exception to this was that blpY and blpZ were missing in Category 4, having been replaced by the remnants of an insertion sequence (IS) element.
This gene is missing from S. flexneri (Sf301) and S. boydii (Sb227) because of inversion-associated deletions, and in Sd197 and S. sonnei (Ss046) it is inactivated by a frameshift mutation and an insertion sequence, respectively (12 ).
In detail, an erythromycin esterase gene (ereA) was detected in a class 2 integron containing its own promoter and capable of being propagated by a class 2 integron with an insertion sequence element (IS 1) upstream of the intI2 gene [ 59].
The yraJ gene, which encodes an outer membrane usher protein in other enterobacteria, was disrupted by an IS2 insertion sequence in S. flexneri 2a str.301.
There were two copies of IS6100 elements at both ends of the three transposon-like entities except that the upstream flanking IS6100 on TnCar was disrupted by a novel insertion sequence ISSsp1.
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