Sentence examples for by an initial denaturation from inspiring English sources

For small DNA fragment amplification, PCR reactions were performed by an initial denaturation step at 94°C for 2 min followed by 30 cycles of denaturing at 94°C for 30 sec, annealing at 56°C for 1 min, extension at 68°C for 9 min, to end with a 10 min extension at 72°C.

PCR reactions were performed by an initial denaturation step at 94°C for 2 min followed by 30 cycles of denaturing at 94°C for 30 sec, annealing at 56°C for 1 min, extension at 68°C for 9 min, to end with a 10 min extension at 72°C.

PCR reactions were started by an initial denaturation at 98°C for 1 min followed by 35 amplification cycles (98°C for 6 s, 10 s at annealing temperature, 72°C for 20 s) and a final extension step for 1 min at 72°C.

The PCR amplification conditions were performed by an initial denaturation step at 94 °C for 10 min followed by 30 denaturation cycles at 94 °C for 1 min, annealing at 60 °C for 1 min and an extension at 72 °C for 1 min followed by a final extension step at 72 °C for 10 min.

DNA was amplified by an initial denaturation at 94°C for 5 min followed by 30 cycles of 30 seconds at 94°C, 1 min at 56°C and 30 seconds at 72°C.

TaqMan Gene Expression Assays (Applied Biosystems) were used in one-step RT-PCR reactions (iScript One-Step RT-PCR Kit, Bio-Rad) consisting of a 50C, 10 min reverse transcription step, followed by an initial denaturation step of 95C for 5 min, and 45 cycles of 95C for 15 sec and 55C for 30 sec.

PCR was performed by using an initial denaturation step at 95 °C for 15 s, followed by 35 amplification cycles of denaturation (95 °C for 1 min), annealing (54 °C for 30 s), elongation (72 °C for 1 min), and a final extension step at 72 °C for 10 min.

The Real-time PCR analysis was performed by using an initial denaturation step at 95°C for 3 min, followed by 50 cycles of denaturation at 95°C for 1 s, annealing at 50°C for 10 s, elongation at 72°C for 8 s.

Thirty-two samples were run in parallel by performing an initial denaturation at 95°C for 8 minutes, and then the PCR reactions were cycled 35 to 40 times as follows: 15 seconds at 95°C, 7 seconds at the appropriate annealing temperature (Table 2), and 18 to 64 seconds at 72°C according to the length of the target sequence annealing (40 s at 58°C).

Polymerase chain reactions (PCR) were carried out by using standard PCR cycle conditions recommended by the manufacturer (an initial denaturation step at 95°C for 10 minutes, followed by 40 cycles of 95°C 15 seconds and annealing at 60°C for 1 minute).

The selected DNA fragments were amplified by PCR with an initial denaturation at 95°C for 1 min, followed by 25 cycles of 94°C for 40 s, 60°C for 1 min, and 72°C for 2 min, and a final extension cycle at 72°C for 5 min. The resulting fragments were cloned into a pGEM-T vector (Promega Corporation, Madison, Wisconsin, USA), inserted into competent XL1-Blue E. coli, and incubated.