Sentence examples for by an expression vector from inspiring English sources

This strain, when transformed by an expression vector containing an operon bglA I- bglT cloned from S. baltica OS155 gained the ability to grow on minimal medium with cellobiose as the sole carbon and energy source.

In order to examine the processing of proguanylin in vitro, we have generated large quantities of the properly folded prohormone by constructing an expression vector that directs its secretion into the periplasmic space of Escherichia coli.

In cultured cells that normally lack this Ca2+ responsiveness, we confer Ca(2+ -mediated activation of a CRE-containing promoter by introducing an expression vector for Ca2+ -mediatedn-dependent protein kinactivationV (CaMKIV).

The clotting factor VIII (FVIII) and cystic fibrosis transmembrane conductance regulator (CFTR) cDNAs have dramatically reduced levels of expression compared to clotting factor IX (FIX) and other cDNAs (100 and 1,000-fold 1,000-foldpectivelowerwhen prespectivelyells by using an expression vector.

We next tested this strategy by electroporating an expression vector encoding both mCherry and guide RNA, a plasmid for Cas9-2A-GFP, and the ssODN template all together into patient iPSCs.

We also activated the mitogenic Ras signaling pathway in our non-neoplastic Barrett's cells by introducing an expression vector containing oncogenic H-RasG12V.

The system employed an epithelial cell background as the virus producing cell and virus production was induced by transfecting an expression vector encoding a viral immediate protein BZLF1 [31], [31].

By using an expression vector, a 680 bp PSA promoter isolated from a prostate cancer patient demonstrated the prostate-specific expression of the reporter gene [ 9].

The effect of the modified 5'ULR on the translation efficiency of iaaM mRNA has been evaluated by using an expression vector with either its original 53 bp or the modified rolA intron sequence preceding the AUG start codon of the DefH9-iaaM gene (Fig. 4, panel a).

Since E-cadherin was lost and underexpressed in the resistant cells, it was transiently expressed in HT29 sensitive and resistant cells by means of an expression vector (pBATEM2-CDH).

In clonal stable transformants established from human immortalized fibroblasts by transfection of an expression vector of Hic-5, the constitutive level of c-fos mRNA was well correlated with that of Hic-5.