To identify Rac1-GEFs that mediate Rac1 activation induced by TNF-α, we performed an expression screen of Rac1-GEFs in endothelial cells and found that the expression of the GEF Trio was up-regulated under inflammatory conditions both in vitro and in vivo.
A great deal of excitement was generated by the recovery of PrPC from an expression screen for soluble Aβ oligomer binders, particularly as synthetic soluble Aβ oligomers were found to damage hippocampal LTP in a PrPC-dependent manner (Lauren et al, 2009) and impairment of spatial memory was rescued by genetic ablation of PrP in a mouse model of AD (Gimbel et al, 2010).
However, a new study by Lechner et al (2009) has defined the developmental acquisition of mechanosensitivity by DRG neurons and, by the use of a new mRNA expression screen, concluded that we almost certainly need to move beyond ASICs and TRP channels in our search for the elusive force-transducing ion channels of this system.
Originally, metagenomics studies depended on the cultivation of clonal cultures followed by functional expression screening [1], which are not able to represent the total community profile and may overlook the vast majority of the microbial biodiversity [2].
They then isolated RANKL by direct expression screening and found, like Wong and coworkers [ 13] did, that it increased dendritic cell stimulated naïve T cell proliferation and survival of RANK-expressing T cells.
To identify such genes, we undertook a large-scale differential expression screen followed by promoter-fusion analysis to detect transcription-factor genes transcribed in the Arabidopsis female gametophyte.
In the present study, we isolated Bcl-XL as an inhibitory gene of TRAIL-induced apoptosis from cDNA library by expression screening assay.
Signal sequence trap by retrovirus-mediated expression screening (SST-REX) was performed as previously described [6], [9].
By gene array expression screening, we found that MeCP2 mRNA and protein were significantly downregulated in response to TAC and were normalized after removal of the aortic stenosis (reversible TAC [rTAC]; Figure 1B and 1C).
In the present study, we set out to discover agents not known for targeting lung adenocarcinoma by an expression-based in silico screening.
