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In all participants, we measured IgG anticardiolipin (Cambridge Life Sciences, UK), IgG aβ2GPI (Corgenix, Denver, USA), β2GPI-oxidised low-density lipoprotein (β2GPI-oxLDL) complex and IgG anti-β2GPI-oxLDL by previously described immunoassays [2, 3], and PAF-AH by an established method [4].
DRM samples were prepared by an established method [34], [35].
Chromosomes were prepared in agaraose plugs from selected His+ recombinants by an established method [82].
A traction field was then calculated from the displacement field by an established method [54].
Platelets were isolated by an established method that limits contamination from other blood cells as described previously [14].
The content of antibiotic entrapped in liposomes (after disruption with 0.2% Triton X-100) was measured by an established method as described previously for tobramycin and polymyxin B [51], [58].
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One of the important elements in the approach is to identify literature co-citations of human genes and their associated GO and MeSH terms by using an established method in text mining [ 19].
Sampling by telephone is an established method [23].
Assessment of predictive value of WT1 mRNA transcript quantitation was based on sequential measurements by qRT-PCR, an established method.
To validate the properties of OliCheck by comparison with an established method, we generated 60-mer oligonucleotides probes with homogenous thermodynamic criteria for N315 genome by the recently published ArrayOligoSelector [ 13] tool.
Heteroplasmy quantification was performed by ARMS-qPCR, an established method in the field (Steinborn et al., 2000; Paull et al., 2013; Tachibana et al., 2013), as described in Burgstaller et al. (2014).
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