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The cells were examined by an epifluorescence microscope (NIKON Eclipse) using separate filters for nuclei, DAPI filter (blue), and for QD (620); TRITC filter (red).
Images were visualised by an epifluorescence microscope (Zeiss, Oberhochen, Germany) and analysed using an Applied Imaging CytoVision Work station (Newcastle, UK, USA).
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The intracellular lipids were examined by using an Epifluorescence Microscope (Nikon DS-Ri1) at the wavelength of 540 nm.
The principle of the immunolocalization technique in plants basically involves fixation and permeabilization of cells, the use of monoclonal or polyclonal antibodies attached to a signaling molecule, usually a fluorochrome and detection of the target molecule by using an epifluorescence microscope.
Pictures of the tissue sections were taken by using an epifluorescence microscope (Keyence, BZ-9000, Neu-Isenburg, Germany) with filters for visualization of DAPI and TRITC and a × 40 objective (CFI Plan Apo), and rolling circle products (RCPs) were counted.
Finally, after washing with PBS pH 7.4, sections were air-dried and mounted with VECTASHIELD mounting medium (Vector, Burlingame, CA, USA) and analyzed by a blinded observer in an epifluorescence microscope.
Images were taken by a CCD camera attached to an epifluorescence microscope (Axioskop 2, Carl Zeiss AG, Germany).
High-resolution microscopic examination of the same tissues could be done by a relatively inexpensive modification of an epifluorescence microscope using a custom designed diode laser light source.
For each condition approximately 30 worms were observed under an epifluorescence microscope, and 5 were imaged by confocal microscopy.
GFP expression in embryos was detected using an epifluorescence microscope and GUS expression in embryos was detected by histochemical staining.
The interaction frequency was calculated by counting the number of YFP positive nuclei among all protoplasts under an epifluorescence microscope (Olympus, MVX100).
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