Sentence examples for by an enzyme solution from inspiring English sources

Root tips were then washed twice with deionized water, digested by an enzyme solution (2% cellulose, 5% pectinase, 4 mM citric acid, 6 mM sodium citrate) for 10 min at 37 °C, and then squashed on a glass slide.

Prefermentation in corncobs hydrolysate was initiated by adding yeast and an enzyme solution corresponding to 3 FPU gWIS-1.

On passaging, the cells were detached from the flask by incubation with a mixture of an enzyme solution of 0.125% trypsin(Gibco) and 0.02% ethylenediaminetetraacetic acid (EDTA, Gibco) for 2 5 min at room temperature.

Aspergillus niger was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate.

Physical adsorption method can be achieved by mixing enzyme solution and solid support for a period of time and then removing unabsorbed enzyme by washing with buffer [ 25– 29].

Spectrophotometric laccase activity assays were routinely carried out in a 96-well plate at 25°C with 0.5 mM ABTS in McIlvaine buffer at pH 4 (mixture of 0.1 M citric acid and 0.2 M K2HPO4) using a Varian Cary 50 Bio or a Bio Tek Synergy Mx spectrophotometer and initiated by adding enzyme solution.

We confirmed xylose yield improved by adding enzyme solution of YKX1, in saccharification experiments using rice straw (data not shown).

The reaction was initiated by adding enzyme solution (75 μl) to the incubation medium.

The pump was stopped after 10 min when the buffer solution was completely replaced by the enzyme solution.

To demonstrate that reaction times can be controlled at will in our screening system, we performed a discontinuous assay by compartmentalizing PAS enzyme solution (crude lysate of cells expressing wild-type PAS) along with substrate in droplets and heat inactivating the enzyme after chosen assay times.

The effect of metal ions on the activity of the phytase was studied by incubating metal ions with a purified enzyme solution (0.5 ml) for 10 min at 55°C.