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Plasma glucose was measured by an autoanalyzer using the potassium ferricyanide method (Technicon Instruments, Tarrytown, NY).
Serum uric acid was determined by an autoanalyzer using the phosphotungstate method.
Plasma glucose was measured by an autoanalyzer using standard enzymatic methods (Randox Laboratories; South Africa) and insulin was measured by Immulite (Siemens Chemiluminescent Technology).
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Plasma glucose was determined on an autoanalyzer using the glucose oxidase method.
Serum glucose was measured in duplicate on an autoanalyzer using the hexokinase method.
Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were determined with an autoanalyzer using audit diagnostics AST and ALT.
Plasma glucose was measured on an autoanalyzer using the glucose oxidase method (YSI model 2300; Yellow Springs Instruments, Yellow Springs, OH).
Creatinine was measured by a Hitachi autoanalyzer using a modified kinetic Jaffe reaction.
The creatinine concentrations in urine were determined kinetically by a clinical autoanalyzer using the Jaffe reaction (Larsen 1972).
The blood sugar was assayed by autoanalyzer using oxidase peroxidase method.
CRP was measured by Cobas Integra 400 autoanalyzer using a particle enhanced turubidimetric assay (Cobas Integra C-Reactive Protein Latex, Roche Diagnostics, Indianapolis, IN).
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