In addition, as with previous experiments fold differences between fetal and NEC cells were compared by assigning an arbitrary fold value of one to fetal cells before comparing with NEC cells.
As with other studies, an arbitrary fold level of one was chosen for A-20 mRNA and protein in controls compared to post-knockdown.
To identify cell cycle-regulated proteins, an arbitrary fold change cutoff of 2.0 (1.0 in the log2-transformed axis) was set, as indicated by the border between the orange and blue boxes.
Genes were selected for validation based on an arbitrary fold change cutoff of 2.5 and those genes containing regulatory elements that are activated by TSPs such as p53 and FOXO were identified (Table 7).
Because mRNA was extracted from whole flies, the signal of many potentially interesting transcripts may have been diluted and consequently missed by assigning arbitrary fold change cutoff values when we evaluated the data.
The input for a GSEA was a list of genes or proteins that were sorted by an arbitrary criterion (e.g., fold changes of expression values).
The adoption of real-time qRT-PCR methodology is somewhat reminiscent of the introduction of cDNA expression microarrays in that initial microarray studies did not identify differentially expressed genes by a statistical method, but by an arbitrary cut-off value of fold-change [ 12].
To bypass this bias probably originating from the amplification step, we set an arbitrary 10 fold expression-threshold to the 37,818 contigs obtained from both sequenced samples.
Results are presented as arbitrary fold.
An annotation may be referenced by an arbitrary number of spatial entities and vice versa.
