Sentence examples for by amplification of partial from inspiring English sources
HPV16 (L1 n = 14, LCR n = 22), HPV31 (L1 n = 4, LCR n = 8), and HPV58 (L1 n = 8, LCR n = 5) found in cervical samples were further characterized by amplification of partial sequence of L1 and LCR by means of the specific primer pairs described in Table 1.
Nested reverse transcription PCR and hemi-nested reverse transcription PCR were used for amplification of partial nucleocapsid gene sequences from human and rodent samples, respectively, by using primers described (11 ).
Amplification of partial 16S rRNA genes of bacteria other than anammox was also influenced by the annealing temperature selected.
These primers did not allow amplification of partial rpoB gene for the 6 M. phocaicum strains.
Total RNA was extracted from rodent lung tissues by using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions and subjected to RT-PCR for amplification of partial hantavirus small (S) segment sequences.
For amplification of partial fibre gene sequences, published primers based on the conserved regions of this gene were used [ 4].
A putative partial MMP sequence was identified by sequencing several of the cloned PCR products and completed by amplification of the 5'-end of the cDNA using specific primers.
Among dogs, 80.9% (76/94) were positive for hookworms by microscopic examination, and 95.7% (90/94) were positive by PCR based on amplification of the partial ITS gene.
The prevalence of hookworm infection among the 218 persons tested in Dong village was 26.6% (58/218) as determined by microscopic examination and 57.4% (124/218) as determined by PCR based on amplification of the partial ITS gene.
A. phagocytophilum was detected in questing and feeding I. ricinus ticks by using a nested PCR amplification of the partial 16S rRNA gene (546-bp fragment) as described (4, 5 ) and in rodent blood by using a real time-PCR assay targeting the msp2 gene (77 bp) (6 ).
In the cell culture supernatant analysis by the RDV method, the specimen exhibited amplification of the partial nucleotide sequences of coxsackie A14 virus (nucleotide sequence data are available in the DDBJ/EMBL/GenBank databases under accession nos. AB275848 AB275853).
