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We have confidence in the KoRV sequences reported here since i) in some cases the sequences match exactly with previously published data; and ii) the assembly is supported by alignments of sequence reads back to the transcripts (analysis not shown).
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This transition is represented in the alignment of Figure 2. All these substitutions were silent as shown by alignment of sequence R in BLASTX (NCBI; not shown).
The apicoplast genome sequence (type I strain RH) was from GenBank (acc# NC001799) and the mitochondrial genome sequence was inferred by alignment of sequence fragments (D. Shanmugam and L. Peixoto, unpublished data).
It is noteworthy, that all the nucleotide differences observed between this new strain and the reference strains of genotype1 in nucleotide BLAST were synonymous as shown by alignment of sequence R in BLASTX.
In array development projects, SNPs are discovered by alignment of sequences from a detection panel to a reference genome.
Phylogenetic analysis was performed by alignment of sequences using Clustal W [ 75] and neighbor-joining trees were generated with 1 000 bootstraps.
Both maximum parsimony and maximum likelihood recovered a tree lacking a clade that is robustly supported by morphological data and also by alignments of other sequences (16SrDNA and HSP90).
For both the alpha amylase gene and the aldehyde reductase gene we eliminated the possibility that variations were due to sequencing error by inspecting alignments of sequence reads to the predicted transcripts (analyses not shown).
The L er-0 allele was subjected to dideoxy sequencing by the ABI-Sanger instrument followed by alignment of the sequence trace to the iteratively resolved indel for validation purposes.
Swelfe identifies repeats by alignment of DNA sequences, amino acids sequences and three dimensional (3D) structures.
Unrooted, neighbor-joining tree was constructed by alignments of AGO protein sequences from the indicated plants.
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