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Protein-coding and ribosomal RNA genes were firstly identified using BLAST searches at GenBank, and then by alignment with previously published mt genomes from species of Crassostrea, Mytilus and other closely related mollusks [ 33].
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The stx1c sequences obtained in this study were compared by using BLAST alignment with previously sequenced stx1c (AJ312232), stx1d (and70851), and stx1 (M19473) subtypestypesubtypes
The criteria for predicted mRNA target genes by alignment with each miRNA were based on those previously reported [ 62, 63].
Adapter-containing and low-quality reads were trimmed, followed by alignment with TopHat.
The introns were identified by alignment with the cDNA sequence.
Most "double-hits" are confirmed by alignments with ARPAnno.
The sequences were aligned with previously published OPV sequences from GenBank by using ClustalW (12 ), and the alignments were manually verified by using MEGA 4.0 software (http://www.megasoftware.net).net
Based on the Synchrotron Radiation (SR) beamline optical element-by-element alignment with analysis of the alignment results an optimized beamline alignment algorithm has been designed and developed.
Alignments with the previously published HAdV-B11K-CD46 HAdV-B11K-CD46 HAdV-B11K-CD46ergy minimised to obtain the lowest energy state of the complex34,41.
Genetic variation was assessed throughout the rRNA promoter region used for bisulfite analysis by alignment of genomic DNA with the previously published rRNA gene promoter sequence using freely available software (CLC Workbench, CLC bio).
They do this by allowing the alignment of previously identified secondary structural elements with respect to each other.
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