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Prediction of the PfSERCA domains was performed by alignment with a set of other eukaryotic serca (P.
Finally, samples were quantified by alignment with a standard curve generated from serial dilutions of affinity-purified antibody product.
In one large-scale 5' RACE experiment, only 57% of clones generated sequences that were sufficiently long and unambiguous to be identified by alignment with a gene transcript [ 9].
In order to reduce the immunogenic potential of rat αD11 [ 25] in the dog, changes were made to the heavy and light chain variable domain framework sequences by alignment with a matrix of predicted protein sequences encoded by expressed canine IgG cDNA sequences.
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We have demonstrated the suitability of short-read sequencing for de novo assembly and annotation of genes without prior genome information, as well as its reliability and complementarity by alignment with sequence from a close, but distinct, species.
Caty_1.0 transcript prediction CDS sequences were screened against the de novo RNA-seq assembly transcript models by alignment with BLAT (v.34) and an aligment score was calculated as the number of matching bases minus the number of CDS sequence bases missing in alignment gaps normalized by the CDS sequence length.
Therefore, neighbouring clones were joined by alignment with subject sequences obtained by a BLAST search.
The M. anisopliae MAT1-2 HMG-box domain [Pfam: PF00505] was also confirmed by alignment with other MAT1-2 sequences and also carries a conserved intron [ 22, 24].
The rRNA gene boundaries were interpreted as the end of a bounding tRNA gene and by alignment with homologous gene sequences from other insect taxa.
The forward primer of each pair was designed to span a predicted intron/exon splice junction in the pig gene (predicted by alignment with the human RefSeq sequence).
Adapter-containing and low-quality reads were trimmed, followed by alignment with TopHat.
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