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In array development projects, SNPs are discovered by alignment of sequences from a detection panel to a reference genome.
Phylogenetic analysis was performed by alignment of sequences using Clustal W [ 75] and neighbor-joining trees were generated with 1 000 bootstraps.
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This transition is represented in the alignment of Figure 2. All these substitutions were silent as shown by alignment of sequence R in BLASTX (NCBI; not shown).
The apicoplast genome sequence (type I strain RH) was from GenBank (acc# NC001799) and the mitochondrial genome sequence was inferred by alignment of sequence fragments (D. Shanmugam and L. Peixoto, unpublished data).
It is noteworthy, that all the nucleotide differences observed between this new strain and the reference strains of genotype1 in nucleotide BLAST were synonymous as shown by alignment of sequence R in BLASTX.
We have confidence in the KoRV sequences reported here since i) in some cases the sequences match exactly with previously published data; and ii) the assembly is supported by alignments of sequence reads back to the transcripts (analysis not shown).
Swelfe identifies repeats by alignment of DNA sequences, amino acids sequences and three dimensional (3D) structures.
The specific sequences were selected by alignment of all sequences available for each virus from the National Center for Biotechnology Information (NCBI) GenBank Database.
Functional characterization is usually performed by alignment of the sequences to already annotated proteins to find their homologous sequences [ 62, 117 119].
DG markers, identified by comparing DG sequences derived from parental lines, genetically mapped with high fidelity to locations in the genome predicted by alignment of DG sequences to the reference genome.
The location of DG markers on each chromosome was determined by alignment of DG sequences with the reference genome.
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