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Primer specificities were checked by alignment between cDNAs and by BLAST against NCBI databases.
GO annotation were performed by alignment between genes and GO database.
Initially, raw data were first processed by ChromaTOF software 2.0 (Leco Instruments), and further baseline correction, accurate mass calculation, data smoothing and noise reduction, followed by alignment between chromatograms were performed using the MetAlign software [ 32].
In short, this software performs a baseline correction, accurate mass calculation, data smoothing and noise reduction, followed by alignment between chromatograms, generating data files that are reduced in size 100-1000 times.
The DNA sequence similarity among MITE01 and BmHel-2 sequences were estimated by alignment between O. nubilalis from GenBank accessions AB597304.1 (positions 1429 to 1179) and EF396411.1 (685 to 943), and B. mori accessions AP009003.1 (89798 to 90133) and AP009031.1 (54931 to 54604) using the ClustalW algorithm (Gap penalty = 10; Gap Extension - 0.2).
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NCBI's UniGene contains gene-oriented clusters of transcript sequences obtained by alignments between transcript sequences and genomic sequences originating from the same gene.
Wheat gene structures (exon-intron junction sites) were predicted by alignment assembly between 12 rice contig sequences (Pseudomolecules) assembled with a minimum tiling path of 3,408 BAC/PAC clones and rice cDNA models containing the untranslated region but no intron sequences.
Target mutation sites were selected by structural alignment between B. circulans xylanase and other xylanases having different pH optima.
The hole resonant tunneling condition can be obtained by the alignment between the confined levels at the accumulation layer (2DHG) and QW.
The majority of various types of slope movements on the so-called Front Slope (E facing) and Back Slope (W facing) are influenced by the alignment between topography and joints.
The purpose of Study 2 is therefore to present the most recent – albeit incomplete – state of current EFL educational policy by investigating alignment between goals, classroom practices, and student proficiency using evidence available at the time of writing.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com