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Batch effects were removed by aligning the within-batch medians for all measurements.
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Despite the batch and seasonal variations in absolute levels of 25(OH D, each of the within-batch coefficients of variation ranged from 5 to 10%, and thus ranking by 25(OH D status was acceptable.
The mean of the within-batch coefficient of variation for the seven batches was 8.9%.
The within-batch coefficient of variation was < 10% and between-batch coefficient was < 15%.
The within-batch coefficient of variation was 2.1%.
The within-batch precision was <6% in both experiments.
The within-batch coefficient of variation (CV) was 3.84% and the between-batch CV was 2.31%, as reported previously [ 15].
Each batch included two or three duplicate mammograms to allow estimation of the within-batch intraclass correlation coefficient.
The spectra exported from DataAnalysis were aligned through in-house software; the data were stored in a matrix by aligning all the peaks within an error of 1 ppm.
Furthermore, the between batch variability is considerably larger than the within batch variability.
The within batch average %CVs were 4.3 and 5.9, respectively.
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