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VERSE recruited the viral reads by aligning the raw simulated reads to the UCSC hg19 sequence.
By aligning the raw reads in each segment back to the reference assembly with bwa [ 23], 98.7%, 99.3%, 99.1% and 99.2% of reads for the four segments aligned.
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Transcript abundance in silk glands, cephalothorax, and venom glands was estimated by aligning the processed raw paired-end sequence reads from each tissue-specific library to the final non-redundant transcriptome using RSEM [ 63].
Pin two webbing/buckle pieces on the bottom raw edge of each front piece at the placement marks, aligning the raw edges.
Pin the lower/side body and upper body with the right sides together, aligning the raw edges.
TMAP is optimized for Ion Torrent™ sequencing data for aligning the raw sequencing reads against a custom reference sequence set containing all transcripts targeted by the AmpliSeq kit.
All SNPs interspaced by less than 5 bp to another SNP or an indel were filtered away as well as SNPs found when aligning the raw C. griseus reads to the C. griseus genome.
Face the right sides together, aligning the raw edges.
By comparison, on the same hardware, GATK takes approximately 24 hr for each exome, using a similar number of parallel threads, plus approximately 2 3 hr more, depending on the number of reads, to align the raw reads to the genome.
Burrows-Wheeler Alignment (BWA) was used to align the raw reads to the reference genome (hg19) (Li and Durbin, 2010).
The score ratio is defined as the ratio of the raw BLAST score computed by aligning a pair of sequences to the raw score of each sequence against itself (i.e. maximum score).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com