Sentence examples for by affinity selection using from inspiring English sources
Through application of our algorithm, it was possible to match 20% of the polyserum-selected peptides to the database of peptides isolated by affinity selection using monoclonal antibodies against the virus envelope protein.
The phage repertoire was panned by affinity selection using immunotubes (Nunc; Maxisorp) coated with antigen nmAb-KT [ 7, 11].
We show here display and efficient affinity selection using two alternative capsid proteins, pVII and pIX.
Purification of OPCs was performed using a two-step approach of negative and positive affinity selection using surface markers.
One method, termed Kunkel mutagenesis, is frequently used for constructing libraries of peptide or protein variants in M13 bacteriophage, followed by affinity selection of phage particles.
The system was initially designed to isolate antigens by affinity selection for mass spectrometry analysis.
From such a library, polypeptides with affinity for another molecule can be isolated by affinity selection, panning.
In this study, we hypothesized that an scFv isolated by affinity selection from a phage library can be effectively isolated with high affinity toward a hapten.
The library is then screened by affinity selection against an immobilized binding partner, and through several rounds peptides with high affinity for the target can be identified.
Plasma cells were further isolated by positive magnetic selection using CD138 selection beads (Miltenyi Biotech).
