Sentence examples for by affinity purification using from inspiring English sources
The peptide-specific antibodies were purified by affinity purification using peptide conjugated to AffiGel-10 (Biorad).
The antibodies from rabbit serum were first purified using protein A and protein G columns (GE Healthcare) coupled in tandem followed by affinity purification using a column (Affi-gel 10, Biorad) with covalently coupled human C4BP-PS.
Solubilized HRs were purified by affinity purification using the Ni-NTA (Ni2+-nitrilotriacetate) method.
Nonspecific antibodies were removed through a nonphosphopeptide (KNVKRNKSQRA) column followed by a specific phosphoenrichment of the antibodies by affinity purification using a phosphopeptide column.
Antibodies were purified from serum by ammonium sulfate fractionation followed by affinity purification using CNBr-activated Sepharose (GE Healthcare) with standard protocols.
GS-TAP-tagged DDX3X, isolated by affinity purification using rabbit IgG agarose, was also recruited to the enhanceosome region upon L. monocytogenes infection.
(PDF 11 kb) Additional file 11: Proteins Identified by Tandem Affinity Purification using AIP1 as bait.
All peptides were produced in Escherichia coli by induction (4 h) with 1 mM IPTG, followed by His-tag affinity purification using open column chromatography.
AQP4 protein was isolated by column-based affinity purification using a polyclonal rabbit anti human-AQP4 antibody (Sigma) coupled to cyanogen bromide-activated sepharose 4B according to the manufacturer's instructions (Amersham pharmaci).
By performing an affinity purification using epitope-tagged HP1c we indeed found the euchromatic isoform of HP1 in Drosophila, HP1c, binds to two Zn-finger containing DNA binding factors, Woc and Row [28], which is in marked contrast to HP1a, which does not interact with these proteins.
Internalisation of the EGFR and ER-2 was assessed by biotinylation and affinity purification using a Pin Point Cell Surface Isolation Kit (Pierce, Northumberland, UK) on whole cell lysates followed by western immunoblotting for the EGFR and HER-2.
