Glycosylated hemoglobin (GHb) was determined by affinity chromatography using Gly-Affin GHb columns (Isolab, Inc., Akron, OH, USA).
Vn was thereafter purified by affinity chromatography using heparin as matrix.
Mouse anti-human WNT16B monoclonal antibody was purified by affinity chromatography using a Sephadex G25 gel filtration column (Madison, WI, USA).
NucS protein was purified by affinity chromatography using Ni2+ NTA agarose column followed by an additional step of anion exchange purification in HiTrap Q HP column.
Subsequently, the recombinant proteins were purified by affinity chromatography using mAb against β2m and α3.
The further purification was carried out by affinity chromatography using the soluble homogenized protein.
L. chagasi HBP (HBPLc) was purified by affinity chromatography using heparin agarose column in FPLC automated system.
The 5G8 protein was isolated from G. lamblia trophozoite lysates by affinity chromatography using moAb 5G8-coupled CNBr-Sepharose.
The soluble fraction from the lysate was purified by affinity chromatography using a 5 ml HisTrap FF crude (GE Healthcare) Ni2+-chelating column equilibrated in buffer A (20 mM Tris pH 8.0, 150 mM NaCl, 5 mM imidazole).
14-3-3ε 14-3-3ε 14-3-3ε 14-3-3εy affinity chromatography, uspeciesNi Sepharose 6 Fast FlowereE Healthcare) column and an imidazole gradient ranging from 10 mM to 300 mM imidazole.
Fabs were expressed in HEK293F cells (Thermo Fisher Scientific) and purified by affinity chromatography using a HiTrap LambdaSelect column (GE Healthcare), followed by ion exchange (MonoS; GE Healthcare) and gel filtration (Superdex 200 Increase 10/300 GL; GE Healthcare) chromatography.
