Sentence examples for by affinity capture using from inspiring English sources

Using this strategy, GST-GFP readthrough products (∼53 kDa) could be isolated easily by affinity capture using either glutathione-Sepharose or Strep-Tactin Sepharose.

This step is then followed by affinity capture, using streptavidin-coupled magnetic beads.

Subsequent affinity capture using extract from two other cell lines, HCT116 colon and MCF-7 breast cancer cells, combined with antibody-based detection of XRCC5 and XRCC6 confirmed selective retention of these proteins by GST-RB1N.

These may also include the selected detection of certain peptide sequences after affinity capture using unique PTMs as ligands.

Two rounds of B chromosome-specific cDNA selection were conducted using a method based on previously published selection of hybrids by affinity capture (SHAC) [ 29].

For chromosome-specific cDNA selection we used a protocol based on the previously published selection of hybrids by affinity capture (SHAC) [ 29] technique with some modifications.

Only the proteins identified in a given gel spot that were also identified by affinity capture were reported in this study.

A possible strategy for target identification is biotin-labeling, followed by affinity capture of binding partners in cell lysates.

The complexes were isolated by size exclusion followed by affinity capture on amylose beads in 20 mM Tris, pH 7.9, 150 mM KCl, 0.5 mM EDTA (SCB1).

Ho et al identified six interactions between Ypr015cp and proteins in cell cycle regulation, cell rescue, metabolism by Affinity Capture-MS [ 15]; and Ptacek et al, using proteome chip technology, revealed 13 biochemical interactions in which Ypr013cp is involved [ 16].

We test whether L-GRAAL achieves larger EC by mapping the smaller network to the densest regions of the larger network (the dense regions corresponding to, e.g. large complexes captured by affinity capture-based methods).