Sentence examples for by affinity binding using from inspiring English sources

CD40L+ viruses were isolated from virus preparations by affinity binding, using beads coated with anti-CD40L antibodies.

We have previously considered the time-dependent mass transfer of proteins secreted from cells during microengraving, as well as their accumulation on a substrate by affinity binding, using numerical simulations.

In conclusion, an Hsb-Ag (Hsb-Cap∆41) constructed by affinity binding between SBP fusion protein and HRP-SA can be used in a double-antigen sandwich ELISA.

Neoantigen prediction was obtained using netMHCpan and HLA genotype was determined by Polysolver, Optitype, Phlat and Seq2hla and validated by affinity binding kinetics.

To investigate whether the increased potency of SII Ang II for the mutants D74N, DRY/AAY and N298A was caused by increased affinity for SII Ang II, we studied SII Ang II affinity by heterologous competition binding using tracer amounts of 3H-Ang II and unlabeled SII Ang II.

GST-MP was purified from the soluble fraction obtained after sonication of E.coli BL21 pLysS) cells expressing GST-MP from the pGEX-MP clone by affinity chromatography using GST binding resin as described in the manufacturers protocol.

Consequently, identification of dengue virus binding proteins by affinity chromatography using rE2-DIII will help to understand virus cell entry and to design strategies to block virus infection in the mosquito cells.

We present a structure-based method for predicting class II epitopes that combines molecular mechanics docking of a fully flexible peptide into the MHC binding cleft followed by binding affinity prediction using a machine learning classifier trained on interaction energy components calculated from the docking solution.

Therefore, we decided to probe for thermodynamic synergy between pairs of ESS3b residues by measuring binding affinities using double-cytosine-substituted SL3ESS3 constructs.

In the present study, by a direct measurement of binding affinity using isothermal titration calorimetry (ITC), we show that the affinity of the ATP analogue, ATPγS, for CaMKII increases significantly in the presence of NR2B as shown by the change in value of the association constant, Ka.

Unlike the literature reported by Savory et al., we just found two mutant sequences with slight improvements in the binding affinity by using the proposed strategy in this study.