Sentence examples for by affinity adsorption using from inspiring English sources

The GST variants are expressed in functional form on filamentous phage and variant GSTs with proper affinities are isolated by affinity adsorption, using transition-state analogs as ligands.

GST-HIPK2 was expressed in Reticulocyte Lysate TNT system (Promega) using T7 RNA polymerase in a 2 h-reaction and purified by affinity adsorption to Glutathione-Sepharose (GE Healthcare).

Modular recombinant enzymes are active and free in the reaction mixture at 80 90 °C and deactivated and immobilized by affinity adsorption on cellulose at 40 30 °C.

GST-p27 was produced in Escherichia coli from the plasmid pGEX-5x-3-p27Kip1 (kindly provided by J. Vervoorts-Weber, Institute of Biochemistry, RWTH Aachen University, Germany) and purified by affinity adsorption to glutathione Sepharose.

The non-fouling properties of our LP-PEG coatings were first investigated by monitoring fibrinogen adsorption using QCM-D.

Antibody fragments were purified by affinity chromatography using IMAC.

Antibodies were purified by affinity chromatography using HiTrap Protein G columns (GE Healthcare Life Sciences).

Here we describe an efficient method of purifying PCR products by specifically removing error products, excess primers, and unincorporated dNTPs from PCR product mixtures using flow-through metal-chelate affinity adsorption.

Pyridine adsorption analysis carried out by gaseous adsorption method using conventional hammet method.

Previously, one study concluded that for decontamination of various proteins such as, troponin-I, myoglobin, and creatine kinase, a two-phase extraction using Triton X-114 was noted to provide improved performance relative to affinity adsorption [ 170].

This procedure is known as fuel "laundering" and can be done by an adsorption process using widely available materials.